Ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme.

• Highly sensitive with an intense signal which is easily captured on film or by imaging systems
• Signal duration of 8 hours
• Secondary antibodies included with the product
• Can be stored at room temperature

The substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies

The chemical reaction that occurs between HRP and luminol in hydrogen peroxide produces a weak light that only lasts for a short period of time. SuperSignal substrates contain an enhancer that intensifies the light to an emission wavelength of 425nm and lengthens the duration to at least six hours for easy HRP detection in immunoblot and ELISA applications. These substrates will work on smaller sample volumes while still providing sensitivity to the picogram level or lower. The signal is generated within seconds and produces low background noise. The chemicals used have not been reported to be hazardous and can replace more hazardous radioactive methods of immunodetection to create a safer lab environment and eliminate any additional waste disposal costs. In addition, all substrates are stable at room temperature, eliminating the need to thaw the substrate before running a protocol.

Substrate is stable for at least one year at 4°C (39°F) and 6 months at room temperature.

When combined with optimized antibody concentrations and blocking buffers, the substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.