DetergentOUT™ GBS10 Detergent Removal System, G-Biosciences

Supplier: G-Biosciences

Certificates

Supplier: G-Biosciences

Certificates

About this item

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins

Our DetergentOUT™ GBS10 resin removes free, unbound anionic, nonionic or zwitterionic detergents (e.g. SDS, Triton® X-100 or CHAPS) from aqueous protein and peptide samples with minimal sample loss for downstream analysis by mass spectrometry and other techniques. The DetergentOUT™ GBS10 columns were shown in independent studies to be fully compatible with DI-QTOF and LC-MS/MS. The use of the DetergentOUT™ GBS10 columns significantly increased the number of peptide spectra detected. In addition, the DetergentOUT™ GBS10 columns have a high binding capacity for detergents, i.e. 6mg SDS and 14mg Triton® X-100 by every mL settled resin.

The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionization when analyzed by mass spectrometry.

Ordering information: Accessories not included.

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