ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay

ProteSEEKER™ protease screening assay consists of a ready-to-use dye-labeled protein, which is digested by proteases to release dye-labeled peptides. The absorbance of which is measured for determination of protease activity. The inhibitors are supplied at a 100X concentration and the 1X concentration provides >90% inhibition in most biological samples.

Accessories: Resorufin-Casein Protease Substrate (#786-321): A colorimetric substrate that when treated with proteases releases resorufin that has an absorbance of 570nm. Supplied lyophilized. FITC-Casein Protease Substrate (#786-322): A fluorescent (fluorescein isothiocyanate (FITC)) substrate that when treated with proteases releases FITC that has an excitation at 485nm and emission at 535nm. Supplied lyophilized.

ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.