About this item
The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates.
- Multiple programmable options
- Six-position filter slides
- Linear and orbital shaking
- Temperature control
Users can program endpoint, kinetic, multiple wavelength, linear scan, and area scan measurement types. Easily accessible six-position filter slides enable wavelength selection from 340 to 650nm.
The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. In addition, the instrument features linear and orbital shaking, plus temperature control. Users can program endpoint, kinetic, multiple wavelength, linear scan, and area scan measurement types. Easily accessible six-position filter slides enable wavelength selection from 230 to 700nm.
The FilterMax™ F-Series of readers can operate as standalone instruments or they can be integrated seamlessly with laboratory automation platforms. Units are ideal for a broad range of applications, including drug discovery, genomics, proteomics, and cell-based research. The unique patented design ensures precise performance and sensitivity across all detection modes. Units can use either automated or manual Z-Height Optimization, and can utlize high-density well scanning for unique applications.
The FilterMax™ F-Series of readers are the first microplate readers to employ patented high-power Light Emitting Diodes (LED). The FilterMax™ is able to optimize the signal at the light source for specific dyes or applications, providing high power at critical wavelengths. The Fluorescence Detector Photomultiplier (PMT) operates in photon counting mode, affording increased sensitivity for red dyes and a linear dynamic range over 6 orders of magnitude.
Specifications
- Catalog No:
- 97059-524
- 97059-526
- Wavelength Range:
- 340–650 nm in Fluorescence Intensity-Top Read Mode, 400–650 nm in Luminescence (Glow) Mode, 340–650 nm in Absorbance Mode
- 230–750 nm in Fluorescence Intensity-Top Read Mode, 300–650 nm in Fluorescence Intensity-Bottom Read Mode, 340–750 nm in Time-Resolved Fluorescence Mode, 340–750 nm in Fluorescence Polarization Mode, 400–750 nm Luminescence (Glow) Mode, 230–650 nm in Absorbance Mode
- Accuracy:
- <+1% +10 mOD at 2 OD in Absorbance Mode
- <+1% + 10 mOD at 2 OD in Absorbance Mode
- Detection Limit (384-Well):
- 3 fmol/100 µl fluorescein in Fluorescence Intensity-Top Read Mode
- 0.4 fmol/100 µl fluorescein in Fluorescence Intensity-Top Read Mode, 0.5 fmol/100 µl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 µl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in Fluorescence Polarization Mode
- Detection Limit (96-Well):
- 3 fmol/200 µl fluorescein in Fluorescence Intensity-Top Read Mode, 2 fmol ATP in Luminescence (Glow) Mode
- 3 fmol/100 µl fluorescein in Fluorescence Intensity-Top Read Mode, 25 fmol/200 µl fluorescein in Fluorescence Intensity-Bottom Read Mode, 30 amol/200 µl Europium in Time-Resolved Fluorescence, 3 mP at 10nM Fluorescein in Fluorescence Polarization Mode, 2 fmol ATP in Luminescence (Glow) Mode
- Dimensions:
- 39W x 24H x 58L cm
- 39W x 24H x 58L cm
- Indication Range:
- 0–3.5 OD in Absorbance Mode
- 0-3.5 OD in Absorbance Mode
- Linear Dynamic Range:
- 6 decades in Fluorescence Intensity-Top Read Mode, 6 decades in Luminescence (Glow) Mode
- 6 Decades in Fluorescence Intensity-Top Read Mode, 5 Decades in Time-Resolved Fluorescence
- Measurement Mode:
- Endpoint, Kinetic, Linear Scan, Area Scan
- Endpoint, Kinetic, Linear Scan, Area Scan
- Plate Format:
- 96–384 well
- 6–1536 well
- Read Time:
- 18 sec. on 96-well and 37 sec. on 384-well in Absorbance Mode
- 180sec/96well; 690sec/384well (1sec integration time) in Time-Resolved Fluorescence Mode, 18sec/96well; 37sec/384well in Absorbance Mode
- Read Time (Fly Mode):
- 18 sec. on 96-well and 37 sec. on 384-well in Fluorescence Intensity-Top Read Mode, 18 sec. on 96-well and 37 sec. on 384-well in Luminescence (Glow) Mode
- 18sec/96well; 37sec/384well in Fluorescence Intensity-Top Read Mode, 38sec/96well; 79sec/384well in Flourescence Polarization Mode, 18sec/96well; 37sec/384well in Luminescence (Glow) Mode
- Reproducibility:
- <+0.5% and +0.005 OD at 2 OD in Absorbance Mode
- <+0.5% and +0.005 OD at 2 OD in Absorbance Mode
- Shaking:
- Linear and Orbital
- Linear and Orbital