Specifications
- Particle Size:90 µm
- Description:Phenyl Sepharose™ CL-4B
- Size:200 mL
- Environmentally Preferable:
- pH Range:3 - 12
- Matrix:Cross-linked agarose, 4%, spherical
- Ligand:Phenyl
- Storage Temperature:4 - 30 °C
- Binding Capacity:HSA (Mr 68 000) 15-20 mg/ml drained resin, ß-lactoglobulin (Mr 18 000) 3-5 mg/ml drained resin
- Ligand Density:40 μmole/ml drained medium
- Description:Phenyl Sepharose™ CL-4B
- Flow Rate:150 cm/h
- Cat. No.:95016-996
- Type:Liquid
- Supplier no.:17-0810-01
Specifications
About this item
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
- Phenyl immobilized on 4% cross-linked agarose
- Aromatic ligand
- Stable in all commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride
- Negligible volume variation due to changes in pH or ionic strength
Octyl Sepharose® CL-4B and Phenyl Sepharose® CL-4B are covalently coupled to a cross-linked 4% agarose matrix by ether linkage, resulting in hydrophobic gels with minimal leakage and no ionic properties.
Octyl Sepharose® 4 Fast Flow is another aliphatic media that provides different and complimentary selectivity. It too is made of agarose beads derivatized via uncharged, chemically stable ether linkages.
Octyl Sepharose® CL-4B and Phenyl Sepharose® CL-4B are covalently coupled to a cross-linked 4% agarose matrix by ether linkage, resulting in hydrophobic gels with minimal leakage and no ionic properties.
Phenyl Sepharose® 6 Fast Flow is available with low or high levels of phenyl substitution differing in selectivity, efficiency, and binding capacity. Both low and high sub media are made of highly cross-linked agarose beads derivatized with phenyl groups via uncharged, chemically stable ether linkages.