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Specifications
- Description:PicoPump with hold pressure and vacuum
- Dimensions:43×13×24 cm (17×5.25×9.5")
- Environmentally Preferable:
- Cat. No.:77665-206
- Weight:6.3 kg
Specifications
About this item
Although syringe and piston techniques are popular for microinjection in the nanoliter range, microinjection of sub-nanoliter volumes through a very small opening in a glass micropipette is often better accomplished with a controlled burst of gas pressure. Designed to simplify intracellular injection and a variety of other microinjection tasks, WPI's PicoPumps use precisely regulated pressures for securing cells and injecting them with fluid.
- Inject into a single cell with picoliter volumes
- Regulated Hold, Ejection and Vacuum Pressure
- Carefully regulated air pressures for securing cells and injecting them with fluid
- Independent vacuum regulation
- Hold pressure prevents backfilling of the pipette by capillary action
- PV830 includes vacuum pressure for filling pipettes from the tip or securing floating cells during injection
- Power: 95 to 135 V or 220 to 240 V, 50/60 Hz
Injected volumes range from picoliters to nanoliters. Separate ports supply positive and negative pressure positive pressure for high-pressure ejection, and suction for supporting the cell or for filling the pipette from the tip. In the time between injections, a secondary default 'Hold' pressure is applied to the pipette to prevent fluid uptake through capillary action or diffusion. The pressure pulses are typically controlled by a precision timer.
Timing, ejection pressure, holding pressure and suction are adjusted independently by control knobs and monitored with indicator gauges on the front panel. Injection pressure is controlled by a 20-turn regulator on the front panel. The built-in timing function controls the amount of time that the injection pressure is applied with millisecond resolution. Time intervals can range from 10 s down to 10 ms or less. The pressure burst can also be controlled manually or triggered by an external source.
Specifications:
Pressure input: 0 to 120 psi
Pressure output: 0.3 to 90 psi
Pressure burst timer (10-turn dial): 10 ms to 10 s in Timed mode
Regulator accuracy: 0.1% (20-turn dial)
Regulator repeatability: 0.05 psi (both hold and eject pressures)
Gauge accuracy: 3% at full scale (both hold and eject pressures)
Input connector: Quick Connect (¹/₄" OD tubing)
Output connector: Barbed (¹/₁₆" ID tubing)
Vacuum input: 0 to 30.0" Hg
Vacuum output: 0.2 to 29.9" Hg
Lowest regulated vacuum: 3" water
Regulator accuracy: 0.1% (20-turn dial)
Regulator repeatability: 0.03" Hg
Gauge accuracy: 3% at full scale
Input connector: Quick connect (¹/₄" OD tubing)
Output Connector: Barbed (¹/₁₆" ID tubing)
Control: Manual
Vent: Atmosphere
Fuse (Older models): 120 V: 0.5 A, fast, 0.25×1.25" USA; 230 V: 0.25 A, fast, 0.25×1.25" USA
Fuse (2019 models): 120 V: 0.5 A, fast, 5×20 mm; 230 V: 0.25 A, fast 5×20 mm
Input kit: 10' nylon tubing (0.25" OD, 1000 psi), one ¹/₂" female NPT adapter
Output kit: Two PicoNozzle