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- Description:Coomassie (Bradford) Protein Assay Kit
- Size:630 Tube Assays/3800 Microplate Assays
- Cat. No.:PI23200
- Detectable concentration:100 - 1500 µg/ml
- Supplier no.:23200
The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.
- Stable, ready-to-use kit of the classical Bradford assay reagent
- Colorimetric measure with a standard spectrophotometer or plate reader (595 nm)
- Easy to use, only single reagent; no working reagent preparation required
- Almost immediate color development; add, mix, and read results
- Detects protein concentration in the range 1 to 1,500 µg/mL
- Microplate and cuvette protocols provided with the instructions and adaptable to several target working ranges
When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465nm to 595nm with a concomitant colour change from brown to blue.