EasySep™ Human Memory B Cell Isolation Kit
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Product Details & Documents
Immunomagnetic positive selection of human memory B cells.
- Highly purified human memory B cells isolated in as little as 33 minutes
- No-wash removal of EasySep™ Releasable RapidSpheres™
- Optional isolation of untouched naïve B cells from the same sample
The EasySep™ Human Memory B Cell Isolation Kit is a two-step method designed to isolate CD27+ B cells from fresh or previously frozen peripheral blood mononuclear cells. First, CD27+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD27+ cells, and unwanted non-B cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD27+ memory B cells that are immediately ready for downstream applications. An optional protocol allows for the isolation of naïve B cells in parallel for use in functional studies.
Caution: Product Restrictions: (i) Customer shall not make products or any portion of them, in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier's mark of origin without express written permission of Supplier; and (ii) Customer is familiar with Supplier's restrictions on the use of the Products and shall use the Products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').
Specifications
- Capacity:2×10⁹ cells
- Description:EasySep™ human memory B cell isolation kit
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