- Assay duration:Multiple steps
- Assay Type:Competitive
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Mouse
- Target protein:SCT
- Size:1 Kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Secretin (SCT) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:2 h
- Shelf Life:12 Months
- Detection Range:12.35 - 1000 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:50 µl
- Sensitivity:4.83 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-CEB075MU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Mouse SCT (Secretin). The assay range is from 12.35 to 1000 pg/ml (Competitive kit) with a sensitivity of 4.83 pg/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
Secretin is a hormone that controls the secretions into the duodenum, and also separately, water homeostasis throughout the body. Acting as an anti-Müllerian hormone it is produced in the S cells of the duodenum in the crypts of Lieberkühn. Its effect is to regulate the pH of the duodenal contents via the control of gastric acid secretion and buffering with bicarbonate. It is notable for being the first hormone to be identified. In humans, the secretin peptide is encoded by the SCT gene. It has recently been discovered to play a role in osmoregulation in the hypothalamus, pituitary, and kidney. Secretin is a linear peptide hormone, which is composed of 27 amino acids and has a molecular weight of 3055. A helix is formed in the amino acids between positions 5 and 13.