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- Assay duration:Multiple steps
- Assay Type:Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Mouse
- Target protein:MUC5B
- Size:1 Kit
- Sample Type:Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Mucin 5 Subtype B (MUC5B) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:0.625 - 40 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.232 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEA684MU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Mouse MUC5B (Mucin 5 Subtype B). The assay range is from 0.625 to 40 ng/ml (Sandwich kit) with a sensitivity of 0.232 ng/ml. There is no detectable cross to reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-Assay: CV <10%, Inter-Assay: CV <12%
Mucins are high molecular mass, highly glycosylated macromolecules that are the major components of mucus secretions. MUC5B is a salivary mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva. It is composed of 14.9% protein, 78.1% carbohydrate, and 7% sulfate.Desseyn et al. isolated MUC5B genomic sequence. They found that the central exon encodes a predicted 3,570-amino acid peptide containing 19 subdomains that can be grouped into 4 larger composite units referred to as 'superrepeats'. Since MUC5B and the related proteins MUC2 and MUC5AC are clustered within chromosome 11p15.5, suggesting that they are members of a multigene family.Desseyn et al. reported that the region 3-prime of the large central exon of MUC5B is composed of 18 exons and spans about 10.7 kb.