- Assay duration:Multiple steps
- Assay Type:Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Mouse
- Target protein:LAMA1
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Laminin Alpha 1 (LAMA1) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:0.78 - 50 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.30 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEA574MU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Mouse LAMA1 (Laminin Alpha 1). The assay range is from 0.78 to 50 ng/ml (Sandwich kit) with a sensitivity of 0.30 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Laminin is a basement membrane protein composed of 3 nonidentical chains, A (400 kDa), B1 (230 kDa), and B2 (220 kDa), which are arranged in a cross-shaped structure. Mattei et al., (1989) used in situ hybridization to localize the human laminin A locus to chromosome 18p11.31. By study of mouse-Chinese hamster somatic cell hybrids, Kaye et al., assigned the gene for laminin A to mouse chromosome 17 and confirmed the assignment of the gene for laminin B2 to mouse chromosome 1. Haaparanta et al., reported the nucleotide sequence of LAMA cDNA. They found an open reading frame encoding 3,075 amino acids. Nissinen et al., reported that the complete human laminin A chain contains a 17-amino acid signal peptide and a 3058-residue A chain proper. The chain has a distinct domain structure with numerous internal cysteine-rich repeats.