This assay has high sensitivity and excellent specificity for detecting Mouse IL5 (Interleukin 5). The assay range is from 15.6 to 1000 pg/ml (Sandwich kit) with a sensitivity of 6.0 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

• High sensitivity and specificity
• Perfect reproducibility and consistency across batches
• Quality control with three-level inspections
• Wide range of targets/species available
• Intra-assay: CV<10%; Inter-assay: CV<12%

IL-5 is secreted by a restricted number of mesenchymal cell types. In its native state, mature IL-5 is synthesized as a 115 aa, highly glycosylated 22 kDa monomer that forms a 40 to 50 kDa disulfide-linked homodimer. Although the content of  carbohydrate  is  high, carbohydrate is not needed for bioactivity.  Monomeric IL-5 has no activity and requires a homodimer for function, which is in contrast to the receptor-related IL-3 and GM-CSF cytokines that exist only as monomers.  Just as one IL-3 and GM-CSF monomer binds to one receptor, one IL-5 homodimer is able to engage only one IL-5 receptor.  It has been suggested that IL-5 (as a dimmer) undergoes a general conformational change after binding to one receptor molecule and this change precludes binding to a second receptor.