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- Assay duration:Multiple steps
- Assay Type:Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Mouse
- Target protein:CTHRC1
- Size:1 kit
- Sample Type:Tissue homogenates, cell lysates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:0.156 - 10 ng/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:0.061 ng/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEN690MU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Mouse CTHRC1 (Collagen Triple Helix Repeat Containing Protein 1). The assay range is from 0.156 to 10 ng/ml (Sandwich kit) with a sensitivity of 0.061 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
CTHRC1 is specifically expressed in vascular calcifications of carotid artery lesions and may contribute to vascular remodeling of injured arteries. The deduced 243-amino acid human protein has an N-terminal signal peptide and a short collagen domain containing gly-X-Y repeats. Northern blot analysis revealed Cthrc1 expression in rat lung and brain, with much lower levels in other tissues examined. In situ hybridization and immunohistochemistry localized rat Cthrc1 mRNA and protein to blood vessel adventitia at 8 and 14 days after balloon injury, with lower levels in developing neointima. Significantly less Cthrc1 mRNA expression was detected 4 weeks after injury. Rat Cthrc1 protein was not detected in normal arteries. Human CTHR1 was not detected in noncalcified plaque regions.