This assay has high sensitivity and excellent specificity for detecting Human SMOC1 (SPARC Related Modular Calcium Binding Protein 1). The assay range is from 0.156 to 10 ng/ml (Sandwich kit) with a sensitivity of 0.056 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

• High sensitivity and specificity
• Perfect reproducibility and consistency across batches
• Quality control with three-level inspections
• Wide range of targets/species available
• Intra-assay: CV<10%; Inter-assay: CV<12%

The mature 408-amino acid protein contains a 26-residue signal peptide at the N terminus, followed by a follistatin-like domain, 2 thyroglobulin-like domains interrupted by a novel domain, and an extracellular calcium-binding domain. Recombinant expression in human cells showed that SMOC1 is a glycoprotein with a calcium-dependent conformation. Circular dichroism spectroscopy showed that SMOC1 has an approximately 40% beta-sheet structure. Northern blot analysis of adult mouse tissues revealed a 3.7-kb transcript with strong expression in ovary, and lower expression in brain, thymus, heart, skeletal muscle, liver, and lung. RT-PCR analysis detected a signal in testis also. Immunofluorescence studies showed that SMOC1 localized to basement membranes both in cultured cells and in mouse skin, kidney, and ovary.