Human GZMB ELISA Kit

This assay has high sensitivity and excellent specificity for detecting Human GZMB (Granzyme B). The assay range is from 0.156 to 10 ng/ml (Sandwich kit) with a sensitivity of 0.059 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.

• High sensitivity and specificity
• Perfect reproducibility and consistency across batches
• Quality control with three-level inspections
• Wide range of targets/species available
• Intra-assay: CV<10%; Inter-assay: CV<12%

Granzymes are serine proteases that are released by cytoplasmic granules within cytotoxic T cells and natural killer cells. Granzyme B is an important mediator of cytotoxic lymphocyte granule-induced death of target cells, accomplishing this through cleavage of Bid and cleavage and activation of caspases as well as direct cleavage of downstream substrates. The serine proteinase GZMB is crucial for the rapid induction of target cell apoptosis by cytotoxic T cells. GZMB enters cells in a perforin-independent manner, predicting the existence of a cell surface receptor(s). Motyka et al. (2000) presented evidence that this receptor is the cation-independent mannose 6-phosphate receptor (CIMPR), also called IGF2R. Inhibition of the GZMB-IGF2R interaction prevented GZMB cell surface binding, uptake, and the induction of apoptosis.

Caution: For research use only. Not for use in clinical diagnostic procedures. Please proper stored each component based on the instruction.