- Assay duration:Multiple steps
- Assay Type:Sandwich
- Conjugate ELISA:Biotin
- Format:Pre-coated
- Host:Rabbit
- Primary antibody reactivity:Human
- Target protein:GUCA2B
- Size:1 kit
- Sample Type:Serum, plasma, tissue homogenates and other biological fluids
- Cross Reactivity:No significant cross-reactivity or interference between Guanylate Cyclase Activator 2B (GUCA2B) and analogues was observed
- Detection Method:Colorimetric
- Time to Results:3 h
- Shelf Life:12 Months
- Detection Range:15.6 - 1000 pg/ml
- Storage Temperature:4 °C for one month (frequent use), −20 °C for one year
- Sample Volume:100 µl
- Sensitivity:5.6 pg/ml
- Regulatory Status:RUO
- Cat. No.:MSPP-SEG292HU
- No. of tests:96 wells
This assay has high sensitivity and excellent specificity for detecting Human GUCA2B (Guanylate Cyclase Activator 2B). The assay range is from 15.6 to 1000 pg/ml (Sandwich kit) with a sensitivity of 5.6 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
Uroguanylin and guanylin (GUCA2A), peptide homologs of the bacterial heat-stable enterotoxins (e.g. the E. coli ST toxin; STa), are endogenous activators of the guanylate cyclase-2C receptor (GUCY2C), which synthesizes cyclic GMP (cGMP), a key component of several intracellular signal transduction pathways. The 16 C-terminal amino acids are identical to uroguanylin, and 8 of 13 C-terminal residues are conserved between GCAP II, uroguanylin, and E. coli STa. The GUCA2B gene also has multiple binding sites for the promoter-specific transcription factors AP1 and AP2, and a cAMP-regulated enhancer element. In the mouse, uroguanylin mRNA is most prominent in the proximal small intestine, whereas guanylin mRNA is predominantly expressed in distal small intestine and colon.