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- Size:20 ml
- Storage Temperature:Room Temperature (Recommended in a cool and dark place, <15°C)
- Appearance:Purple to dark purple clear liquid to cloudy liquid.
- Cat. No.:TCB6104-20ML
This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.
SDS-PAGE Sample Buffer is specially formulated for protein sample preparation based on the Laemmli Method of SDS-PAGE system.
Preparation of non-reducing sample
A protein sample is mixed with the 4X SDS-PAGE sample buffer (3:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 20 μl of 2-Mercaptoethanol per 80 μl of 4X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (3:1) and heating to 95 - 100 °C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Reducing reagents: 2-Mercaptoethanol [for Electrophoresis], DL-Dithiothreitol [for Electrophoresis], etc.