Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Description:Caspase-3/7 Activity plate reader assay kit, red
- Size:100 tests
- Cat. No.:MSPP-100-0922
Specifications
About this item
For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis - 100 tests.
- Z-DEVD-ProRed™ detects caspase-3/7 activity
- Excitation wavelength: 532 nm
- Emission wavelength: 619 nm
- Easily scalable for high-throughput assays
- Verified for use with fluorescent plate readers
Efficiently detect caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis by using the Caspase-3/7 Activity Plate Reader Assay Kit, Red, which includes the fluorogenic substrate Z-DEVD-ProRed™ and an assay buffer. This reagent kit uses a quick and simple staining protocol that is well suited for high-throughput screening and compatible with standard fluorescent plate readers.
Caspase-3 and caspase-7 have a proteolytic substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). When added to apoptotic cells, Z-DEVD-ProRed™ is cleaved by activated caspase-3/7, generating a red fluorescent signal that can be detected by standard plate readers.
Caspases are a family of proteases that play a central role in cellular apoptosis through the cleavage of select proteins, and result in the disassembly of the cell (Thornberry & Lazebnik Science, 1998). This process is switched on by pro-apoptotic signals from death receptors or stimuli such as cytotoxic reagents, which activate the initiator caspases and lead to activation of the effector caspases - caspase-3 and caspase-7 (Thornberry & Lazebnik Science, 1998). Caspase assays are useful for quantifying caspase-3/7 activity in apoptotic cells and for screening caspase-3/7 inhibitors.