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Human Native ACTB (from Phlatelets)
  77538-546
 :  Creative Biomart
New Product
 :  
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Human Native ACTB (from Phlatelets)
  77538-546
 :  Creative Biomart
 :  ACTB-325H
New Product
 :  

 

  • Pack type:
    Vial
  • Conjugation:
    Unconjugated
  • Protein/Peptide Type:
    Native
  • Source:
    Phlatelets
  • Species:
    Human
  • Size:
    25 µg
  • Storage Conditions:
    4 °C
  • Biological Activity:
    The biological activity of non-muscle actin can be determined by its ability to efficiently polymerize into filaments in vitro and separate from unpolymerized components in a spin down assay. Stringent quality control ensures that >85% of the non-muscle actin can polymerized in this assay.
  • Reconstitution Instructions:
    The protein should be reconstituted to 10 mg/ml with 100 µl of distilled water, it will then be in the following buffer: 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl₂, 0.2 mM ATP, 5% (w/v) sucrose, and 1% (w/v) dextran.
  • Endotoxin-free:
    No
  • Carrier-Free:
    No
  • Protease-free:
    No
  • Animal-Free:
    No
  • Protein Synonyms:
    actin, beta
  • UniProtKB:
    P60709
  • Protein/Peptide Name:
    ACTB
  • Purity:
    Protein purity is determined by scanning densitometry of Coomassie blue stained protein on a 12% polyacrylamide gel. Non-muscle actin was found to be >99% pure.
  • Molecular Weight:
    Non-muscle actin has an approximate molecular weight of 43 kDa
  • Endotoxin Level:
    Low
  • Nuclease-free:
    No
  • Grade:
    Ultrapure grade
  • Shipping Temperature:
    20
  • Tested Applications:
    Identification and characterization of non-muscle actin binding proteins,in vitro actin polymerization studies,antibody standard for western blot analysis
  • Cat. No.:
    77538-546