Recombinant Human EPCAM (from HEK293 cells)
77539-390
New Product
:
- Pack type:Vial
- Conjugation:Unconjugated
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:Human
- Size:50 µg
- Storage Conditions:4 °C
- Endotoxin Content:<1.0 EU/μg of the protein as determined by the LAL method
- Biological Activity:Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-his coated plates (1.25 μg/ml, 100 μl/well), approximately >40% will adhere specifically after 30 minutes at 37 °C .
- Reconstitution Instructions:It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 µg/µl. Centrifuge the vial at 4 °C before opening to recover the entire contents.
- Endotoxin-free:No
- Carrier-Free:No
- Protease-free:No
- Animal-Free:No
- Protein Synonyms:epithelial cell adhesion molecule
- UniProtKB:P16422
- Protein/Peptide Name:EPCAM
- Purity:>96% as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC.
- Molecular Weight:The recombinant human EpCAM consists of 253 amino acids after removal of the signal peptide and has a calculated molecular mass of 28.84 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human EpCAM is approximately 36.5 kDa due to glycosylation.
- Endotoxin Level:Low
- Nuclease-free:No
- Grade:Ultrapure grade
- Shipping Temperature:20
- Cat. No.:77539-390