Specifications

• Pack type:
  Vial
• Conjugation:
  Unconjugated
• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Size:
  20 µg
• Storage Conditions:
  4 °C
• Endotoxin Content:
  <1.0 EU/μg of the protein as determined by the LAL method
• Biological Activity:
  Measured by its binding ability in a functional ELISA. Immobilized human EGF hFc at 2 μg/ml (100 μl/well) can bind human EGFR his, the EC50 of human EGFR his is 100 - 500 ng/ml. Captured Cetuximab (IgG1) on proA Chip can bind human EGFR recombinant protein with an affinity constant of 1.07 µM as determined in an SPR assay (Biacore T200). Loaded Cetuximab on ProA Biosensor, can bind Human EGFR protein, His and AVI tag with an affinity constant of 5.81 nM as determined in BLI assay (ForteBio Octet Red384) (routinely tested).
• Reconstitution Instructions:
  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 µg/µl. Centrifuge the vial at 4 °C before opening to recover the entire contents.
• Endotoxin-free:
  No
• Carrier-Free:
  No
• Protease-free:
  No
• Animal-Free:
  No
• Protein Synonyms:
  epidermal growth factor receptor
• UniProtKB:
  P00533
• Protein/Peptide Name:
  EGFR
• Purity:
  >95% as determined by SDS-PAGE. >90% as determined by SEC-HPLC.
• Molecular Weight:
  The recombinant human EGF receptor consists of 630 amino acids and has a calculated molecular mass of 69.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 110 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin Level:
  Low
• Nuclease-free:
  No
• Grade:
  Ultrapure grade
• Shipping Temperature:
  20
• Cat. No.:
  77539-346