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Specifications
- Assay duration:Multiple steps
- Assay Type:Competitive
- Conjugate ELISA:Peroxidase
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:Metanephrine and normetanephrine
- Description:Bi-Neph (metanephrine and normetanephrine) ELISA
- Size:2×96 wells
- Sample Type:Urine
- Detection Method:Colorimetric
- Time to Results:1 h, 45 min
- Regulatory Status:RUO
- Cat. No.:101721-248
- Supplier no.:17BINHUE02UFST
Specifications
About this item
The Bi-Neph (Metanephrine and Normetanephrine) ELISA is for the quantitative determination of metanephrine and normetanephrine in urine.
First metanephrine (metadrenaline) and normetanephrine (normetadrenaline) are quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls, and samples and the solid phase bound analytes compete for a fixed number of antiserum binding sites. After the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a reference curve prepared with known standard concentrations. During the sample preparation, Metanephrine (Metadrenaline) is quantitatively acylated.