Biodyne® Transfer Membranes offer high sensitivity and low background for enhanced detection and resolution. They are ideal for nucleic acid detection.
- Membranes do not crack, shrink or tear when subjected to multiple cycles of hybridisation, stripping and reprobing
- Intrinsically hydrophilic for easy wetting
- Superior performance with radioactive (Biodyne® B membrane) and non-radioactive (Biodyne® A membrane) detection systems
Biodyne® A Membrane (Amphoteric Nylon 6.6): Membrane zeta potential can be modulated by changes in pH. Ideal for single probe or multiple rehybridisations, and applications where background is troublesome.
Biodyne® B Membrane (Positively-charged Nylon 6.6): Pore surfaces are populated by a high density of quaternary ammonium groups. Highest sensitivity for nucleic acid applications.
Biodyne® C Membrane (Negatively-charged Nylon 6.6): Can be derivatised by coupling reactions through the carboxyl groups on the pore surfaces.
Biodyne® Plus Membrane (Positively-charged Nylon 6.6 with high isoelectric point): With certain non-radioactive detection systems, it is more sensitive than Biodyne® A membrane while exhibiting lower background than Biodyne® B membrane. Especially suitable for reverse dot blots and ELISA applications.
Biodyne® Transfer Membranes are resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2 M sodium hydroxide, DMSO, and dimethylformamide. They are not compatible with concentrated formic acid (>50%), hydrochloric acid (>4 M), oxidising agents, and long exposures (days to weeks) to pH <2.