Serum- and BPE-free medium for human small airway epithelial cells cultured at the air-liquid interface.

PneumaCult™-ALI-S Medium is a serum- and BPE-free medium for the culture of human small airway epithelial cells at the air-liquid interface (ALI). Small airway epithelial cells cultured in PneumaCult™-ALI-S Medium undergo extensive mucociliary differentiation to form a cuboidal epithelium that exhibits morphological and functional characteristics similar to those of the human small airway in vivo. Together, PneumaCult™-ALI-S Medium and PneumaCult™-Ex Plus Medium constitute a fully integrated BPE-free culture system for in vitro human small airway modeling. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.