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- Description:STEMdiff™ hematopoietic kit
- Size:1 kit
- Cat. No.:MSPP-05310
For differentiation of human PSCs into the hematopoietic lineage.
Generate functional hematopoietic progenitor cells from human embryonic stem (ES) and induced pluripotent stem (iPS) cells with STEMdiff™ Hematopoietic Kit. Using a simple and reproducible protocol in serum- and feeder-free conditions, you can produce hematopoietic progenitor cells expressing CD34, CD45, and CD43. The robust, two-stage protocol initially induces cells toward the mesoderm before further differentiating them into hematopoietic progenitor cells. After 12 days, a population of hematopoietic cells containing 25 to 65% (average 43%) CD34+CD45+ progenitor cells can be harvested, including progenitor cells that have the capacity to form functional hematopoietic colonies in the colony-forming unit (CFU) assay.
STEMdiff™ hematopoietic kit has been optimized for differentiation of human pluripotent cells maintained in:
mTeSR™1
mTeSR™ plus
TeSR™-E8™