Experience reliable amplification even in GC-rich regions with Radiant™ GC-Long DNA polymerase and GC-Long ultra polymerase.

• New-generation 5X PCR buffer formulation including optimal levels of ultra-pure dNTPs, MgCl₂ and enhancers for maximum PCR efficiency with problematic and long targets
• Robust PCR performance across a wide range of DNA templates including GC-rich templates and Long amplicons of <20 Kb
• Significantly higher fidelity than wild-type Taq Polymerase and an error rate of 5.0×10-5

Radiant™ GC-Long DNA polymerase Radiant™ GC-Long DNA polymerase is a high performance, hot-start complex of enzymes and additives optimized for the sensitive DNA amplification of a wide range of challenging DNA templates including complex mammalian genomic DNA and long PCR amplicons greater than 20 Kb. The Hot-Start technology is based on a new-generation PCR buffer formulation in conjunction with a antibody-mediated chemistry. Radiant™ GC-Long DNA Polymerase exhibits significantly higher fidelity than wild-type Taq with an error rate of 5.0×10 - 5.

The polymerase is ideal for multiplexing, low-copy assays, and the amplification of challenging GC-rich and AT-rich targets as well as long PCR of amplicons >20 Kb. Radiant™ GC-Long DNA Polymerase is engineered for robust, superior PCR and is supplied with a highly optimized, new-generation 5X buffer system which provides exceptional sensitivity and ease of use. Storage Radiant™ GC-Long DNA Polymerase is shipped on blue or dry ice and should be stored at –20 °C upon receipt. Excessive freeze/thawing should be avoided. When stored as specified, Radiant™ GC-Long DNA Polymerase DNA Polymerase is stable for 12 months from date of receipt. The kit may also be stored at 4 °C for 1 month.

Important Considerations Radiant™ 5X GC-Long Buffer: The 5X reaction buffer contains 15 mM MgCl₂, 5 mM dNTPs, PCR enhancers and has been optimized for maximum efficiency, sensitivity and success with difficult amplicons. We do not suggest the use of additional PCR enhancers, dNTPs or MgCl₂. Template: For complex genomic DNA, we suggest the use of 5 to 500 ng per reaction; For cDNA or plasmid DNA, please use <100 ng per reaction. Primers: Primers should have a predicted melting temperature of around 60 °C, using default Primer 3 settings. The final primer concentration in the reaction should be between 0.2 and 0.6 μM. Annealing: We recommend performing a temperature gradient to determine the optimal annealing temperature.

Alternatively, we suggest a 55 °C annealing temperature. Increase in 2 °C increments if non-specific products are present. Extension: Optimal extension is achieved at 72 °C. The optimal extension time is dependent on amplicon length and complexity. 15 seconds per kilobase(Kb) is recommended for amplification from eukaryotic genomic DNA or cDNA up to 5 Kb. For longer amplicons >5 Kb, we recommend between 40 seconds and 1 minute per Kb. Quality Control Radiant™ GC-Long DNA Polymerase is tested extensively for robust activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination. Radiant™ GC-Long DNA Polymerase is manufactured under a comprehensive quality management system, following ISO 9001:2008 standards.