Achieve high-performance PCR results with Radiant™ 2X Blue Taq Supermix.

• Inert blue dye will not inhibit PCR reaction and is used as a downstream gel loading dye
• No change in PCR cycling conditions when in supplied red format
• New-generation PCR formulation including enhancers for maximum PCR efficiency, sensitivity and reaction speed
• Robust PCR performance across a wide range of DNA templates including genomic DNA and GC-rich and AT-rich sequences
• Supplied as a ready-to-use Red 2X Mastermix for maximum convenience and minimal liquid handling
• High-yields with amplicons up to 5 Kb with standard or fast cycling

Radiant™ 2X Blue Taq Supermix RadiantTM 2X Blue Taq Mastermix is a ready-to-use, robust PCR mix containing highly purified Taq DNA Polymerase, reaction buffer, ultra-pure dNTPs, MgCl₂ and PCR enhancers. RadiantTM 2X Blue Taq Mastermix is optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian genomic DNA and is ideal for screening, colony PCR, high-throughput PCR and genotyping of problematic GC-rich and AT-rich sequences. RadiantTM 2X Blue Taq Mastermix is provided at 2X concentration and used at 1X concentration by adding template, primer, and H₂O.

Storage RadiantTM 2X Blue Taq Mastermix is shipped on blue or dry ice and should be stored at –20 °C upon receipt. Excessive freeze/thawing should be avoided. When stored as specified, RadiantTM 2X Blue Taq Mastermix is stable for 12 months from date of receipt. The Kit may also be stored at 4 °C for 1 month. Important Considerations RadiantTM 2X Blue Taq Mastermix: The 2X Blue Mastermix is comprised of RadiantTM Taq DNA Polymerase, inert Gel Loading Buffer, 2 mM dNTPs, 6 mM MgCl₂, and PCR enhancers for maximum efficiency, sensitivity and success with difficult amplicons. We do not suggest the use of additional PCR enhancers.

Template: For complex genomic DNA, we suggest the use of 5 to 500 ng per reaction; For cDNA or plasmid DNA, please use <100 ng per reaction. Primers: Primers should have a predicted melting temperature of around 60 °C, using default Primer 3 settings. The final primer concentration in the reaction should be between 0.2 and 0.6 μM. Annealing: We recommend performing a temperature gradient to determine the optimal annealing temperature. Alternatively, we suggest a 55 °C annealing temperature. Increase in 2 °C increments if non-specific products are present. Extension: Optimal extension is achieved at 72 °C. The optimal extension time is dependent on amplicon length and complexity. 15 seconds per Kilobase (Kb) is recommended for amplification from eukaryotic genomic DNA or cDNA. For shorter amplicons, a 1 second extension is sufficient.

Quality Control RadiantTM 2X Blue Taq Mastermix is tested extensively for robust activity, processivity, efficiency, heat activation, sensitivity, absence of nuclease contamination and absence of nucleic acid contamination. RadiantTM 2X Blue Taq Mastermix is manufactured under a comprehensive quality management system, following ISO 9001:2008 standards.