TBS 10X Solution (Tris Buffered Saline 10X Solution) is a commonly used reagent in life science research. It is a buffered salt solution consisting of Tris (tris(hydroxymethyl)aminomethane) and sodium chloride (NaCl) dissolved in water. TBS 10X Solution is an essential reagent in life science laboratories for maintaining pH stability and providing appropriate ionic conditions for biological experiments.
Using TBS 10X Solution creates a stable pH environment for various biological experiments, such as protein and nucleic acid analysis. Tris serves as a buffering agent, helping to maintain a consistent pH level in the solution, while sodium chloride provides ionic strength to mimic physiological conditions. To dilute TBS 10X Solution to a lower concentration (e.g., 1X) - mix it with distilled water before using it an experiment. This diluted solution can be used for various applications including protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques.
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