Human Recombinant HLA-A*02:01&B2M&MAGE-A3, Biotin

Quality validated and reproducible proteins provided at very competitive pricing.

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Melanoma antigen family A, 3 (MAGE-A3) is a cancer-testis antigen whose expression has been demonstrated in a wide array of malignancies including Melanoma, brain, breast, lung and ovarian cancer. In addition, its ability to elicit spontaneous humoral and cellular immune responses has been shown in cancer patients.

: ISO 13485

: One to two days.

: Recyclable.

Conjugation:
Biotin
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Tag sequence:
C-His-Avi
Storage Conditions:
–20 to –80 °C for 12 months as supplied from date of receipt. –80 °C for 3 - 6 months after reconstitution. 2 - 8 °C for 2 - 7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Endotoxin Content:
Less than 1 EU per μg by the LAL method.
Gene ID:
A0A140T913(HLA-A*02:01)&P61769(B2M)&KVAELVHFL
Reconstitution Instructions:
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Endotoxin-free:
Y
Carrier-Free:
Y
Animal-Free:
N
Protein Synonyms:
HLA-A*02:01&B2M&MAGE-A3 (KVAELVHFL) Monomer
Protein/Peptide Name:
HLA-A*02:01&B2M&MAGE-A3
Purity:
<95% as determined by Tris-Bis PAGE; <95% as determined by HPLC
Molecular Weight:
The protein has a predicted MW of 50.50 kDa. Due to glycosylation, the protein migrates to 50 - 65 kDa based on Tris-Bis PAGE result.
Sequence:
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and KVAELVHFL peptide
Endotoxin Level:
Less than 1 EU per μg by the LAL method.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Shipping Temperature:
Shipped at ambient temperature.
Tested Applications:
SDS-Page, HPLC