Human Recombinant HLA-A*02:01&B2M&PRAME, Biotin (MHC-HM447B)

: Kactus Bio

Human Recombinant HLA-A*02:01&B2M&PRAME, Biotin (MHC-HM447B)

: Kactus Bio

Quality validated and reproducible proteins provided at very competitive pricing.

Best quality
Reproducible supply
Functionally validated
Lowest price

PRAME (Preferentially expressed Antigen in Melanoma) is a Melanoma-associated antigen expressed in cutaneous and ocular Melanomas and some other malignant neoplasms, while its expression in normal tissue and benign tumors is limited.

: ISO 13485

: One to two days.

: Recyclable.

Conjugation:
Biotin
Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Tag sequence:
C-His-Avi
Storage Conditions:
–20 to –80 °C for 12 months as supplied from date of receipt. –80 °C for 3 - 6 months after reconstitution. 2 - 8 °C for 2 - 7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Endotoxin Content:
Less than 1 EU per μg by the LAL method.
Gene ID:
P79603(HLA-A*02:01)&P61769(B2M)&ALYVDSLFFL
Reconstitution Instructions:
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Endotoxin-free:
Y
Carrier-Free:
Y
Animal-Free:
N
Protein Synonyms:
HLA-A*02:01&B2M&PRAME (ALYVDSLFFL) Monomer
Protein/Peptide Name:
HLA-A*02:01&B2M&PRAME
Purity:
<95% as determined by Tris-Bis PAGE; <95% as determined by HPLC
Molecular Weight:
The protein has a predicted MW of 50.60 kDa. Due to glycosylation, the protein migrates to 50 - 65 kDa based on Tris-Bis PAGE result.
Sequence:
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and ALYVDSLFFL peptide
Endotoxin Level:
Less than 1 EU per μg by the LAL method.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS, 100 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Shipping Temperature:
Shipped at ambient temperature.
Tested Applications:
ELISA, SDS-Page, HPLC