Human Recombinant HLA-A*02:01&B2M&P53 WT (MHC-HM416T)

Quality validated and reproducible proteins provided at very competitive pricing.

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p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein. p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.

: ISO 13485.

: One to two days.

: Recyclable.

Protein/Peptide Type:
Recombinant
Source:
HEK293 cells
Species:
Human
Tag sequence:
C-His-Avi
Storage Conditions:
–20 to –80 ℃ for 12 months as supplied from date of receipt. –80 ℃ for 3 - 6 months after reconstitution. 2 - 8 ℃ for 2 - 7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Endotoxin Content:
Less than 1 EU per μg by the LAL method.
Gene ID:
A0A140T913(HLA-A*02:01)&P61769(B2M)&HMTEVVRRC
Reconstitution Instructions:
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Endotoxin-free:
Y
Carrier-Free:
Y
Animal-Free:
N
Protein Synonyms:
P53|HLA-A|BCC7|TRP53|P53 (HLA-A*02:01) Tetramer|MHC|TP53|LFS1|Antigen NY-CO-13|FLJ92943
Protein/Peptide Name:
HLA-A*02:01&B2M&P53 WT
Purity:
<95% as determined by Tris-Bis PAGE; <95% as determined by HPLC
Molecular Weight:
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260 - 265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Sequence:
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRRC peptide
Endotoxin Level:
Less than 1 EU per μg by the LAL method.
Formulation:
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Shipping Temperature:
Shipped at ambient temperature.
Tested Applications:
SDS-Page, HPLC