Specifications

• Protein/Peptide Type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  C-His-Avi
• Storage Conditions:
  –20 to –80 ℃ for 12 months as supplied from date of receipt. –80 ℃ for 3 - 6 months after reconstitution. 2 - 8 ℃ for 2 - 7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Endotoxin Content:
  Less than 1 EU per μg by the LAL method.
• Gene ID:
  A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA
• Reconstitution Instructions:
  Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Endotoxin-free:
  Y
• Carrier-Free:
  Y
• Animal-Free:
  N
• Protein Synonyms:
  Glycoprotein 100|MHC I|HLA0201|gp100|ME20-M|HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer|P100
• Protein/Peptide Name:
  HLA-A*02:01&B2M&GP100
• Purity:
  <95% as determined by Tris-Bis PAGE; <95% as determined by HPLC
• Molecular Weight:
  The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260 - 265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
• Sequence:
  Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide
• Endotoxin Level:
  Less than 1 EU per μg by the LAL method.
• Formulation:
  Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Shipping Temperature:
  Shipped at ambient temperature.
• Tested Applications:
  ELISA, SDS-Page, HPLC