SMART Digest™ Dual Enzyme Columns for HDX-MS Analysis
TS60160-110
- Column Format:Cartridge
- Flow rate:10 - 500 μl/min
- Ø int.:2.1 mm
- Pore size:1000 Å
- Max. Pressure:2500 psi
- Description:Pepsin/Protease XIII dual enzyme column
- Length:30 mm
- pH Range:2 - 6
- Temperature Limit:4...50 °C
- Column hardware:PEEK
- Cat. No.:TS60160-110
- Packing material:Hydrophilic/coated DVB
- Phase:Immobilized enzyme
SMART Digest™ dual enzyme columns containing pepsin and protease XIII provide fast, reliable digestion of proteins for for HDX-MS analysis helping to provide the sequence characterization of biotherapeutics.
- 1-year stability
- pH range for pepsin activity is 1 to 3
- Proteinase XIII is active at low pH
Pepsin: Cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine, tryptophan and tyrosine.
Protease XIII: Cleaves proteins preferentially at carboxylic groups of basic amino acids (arginine, lysine, histidine).