The Pierce™ Far-Western Blot Kit for Biotinylated Proteins provide a nonisotopic approach to far-Western analysis that can be performed either directly in the gel or on the membrane

• Reliable and sensitive detection method offers a practical and safe alternative to radiolabeling the bait protein
• Primary antibody-free detection - uses a biotinylated protein as the probe, eliminating the need for primary antibodies or even to have prior knowledge of target-protein identities
• Compatible with both SDS-PAGE and native gels
• Biotin/streptavidin-HRP systems demonstrate less nonspecific binding compared to antibodies
• Gels can be used for total protein staining after the in-gel chemiluminescent detection step, eliminating the need to run two gels

On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.