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SARS-CoV-2 (2019-nCoV) Recombinant Spike RBD(K417N His Tag) is Recombinant Protein (From HEK293 Cells), Biotinylated
SARS-CoV-2 (2019-nCoV) Recombinant Spike RBD(K417N His Tag) is Recombinant Protein (From HEK293 Cells), Biotinylated
Catalog # 77447-814
Supplier:  Sino Biological
CAS Number:  
SARS-CoV-2 (2019-nCoV) Recombinant Spike RBD(K417N His Tag) is Recombinant Protein (From HEK293 Cells), Biotinylated
Catalog # 77447-814
Supplier:  Sino Biological
Supplier Number:  40592-V08H59-B-20
CAS Number:  

Specifications

  • Conjugation:
    Biotin
  • Protein/Peptide Type:
    Recombinant
  • Source:
    HEK293 cells
  • Species:
    SARS-CoV-2
  • Size:
    20 µg
  • Tag sequence:
    His Tag
  • Storage Conditions:
    Samples are stable for up to twelve months from date of receipt at −20 to −80 ℃. Store it under sterile conditions at −20 to −80 ℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Endotoxin Content:
    <1.0 EU per μg protein as determined by the LAL method
  • Biological Activity:
    Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 μg/ml (100 μl/well) can bind SARS-CoV-2 Spike RBD(K417N)-His Recombinant Protein, Biotinylated (Cat: 40592-V08H59-B), the EC50 of SARS-CoV-2 Spike RBD(K417N)-His Recombinant Protein, Biotinylated (Cat: 40592-V08H59-B) is 20 - 160 ng/ml.
  • Reconstitution Instructions:
    A hardcopy of the datasheet with reconstitution instructions is sent together with the product. Please refer to it for detailed information.
  • Endotoxin-free:
    No
  • Carrier-Free:
    Yes
  • Animal-Free:
    No
  • Protein Synonyms:
    CoV Spike Protein
  • Protein/Peptide Name:
    Spike
  • Purity:
    >85% as determined by SDS-PAGE.
  • Molecular Weight:
    The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 235 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions.
  • Shipping Temperature:
    Ambient
  • Cat. No.:
    77447-814

Specifications

About this item

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation K417N) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.