SARS-CoV-2 (2019-nCoV) Recombinant Spike RBD(K417N His Tag) is Recombinant Protein (From HEK293 Cells), Biotinylated
Catalog # 77447-814
Supplier: Sino Biological
CAS Number:
Specifications
- Conjugation:Biotin
- Protein/Peptide Type:Recombinant
- Source:HEK293 cells
- Species:SARS-CoV-2
- Size:20 µg
- Tag sequence:His Tag
- Storage Conditions:Samples are stable for up to twelve months from date of receipt at −20 to −80 ℃. Store it under sterile conditions at −20 to −80 ℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
- Endotoxin Content:<1.0 EU per μg protein as determined by the LAL method
- Biological Activity:Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 μg/ml (100 μl/well) can bind SARS-CoV-2 Spike RBD(K417N)-His Recombinant Protein, Biotinylated (Cat: 40592-V08H59-B), the EC50 of SARS-CoV-2 Spike RBD(K417N)-His Recombinant Protein, Biotinylated (Cat: 40592-V08H59-B) is 20 - 160 ng/ml.
- Reconstitution Instructions:A hardcopy of the datasheet with reconstitution instructions is sent together with the product. Please refer to it for detailed information.
- Endotoxin-free:No
- Carrier-Free:Yes
- Animal-Free:No
- Protein Synonyms:CoV Spike Protein
- Protein/Peptide Name:Spike
- Purity:>85% as determined by SDS-PAGE.
- Molecular Weight:The recombinant SARS-CoV-2 (2019-nCoV) Spike RBD consists of 235 amino acids and predicts a molecular mass of 26.6 kDa. As a result of glycosylation, it migrates as an approximately 36 kDa band in SDS-PAGE under reducing conditions.
- Shipping Temperature:Ambient
- Cat. No.:77447-814
Specifications
About this item
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) Spike RBD (YP_009724390.1, with mutation K417N) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.