One of the most widely used cell types in research, drug development, and toxicity testing is hepatocytes (liver cells). Existing liver models (for cell culture based systems) include primary human hepatocytes harvested from donors, immortalized cell lines (such as HepG2), and cells from rodent models. Each of these models suffers experimental limitations.

• Cryovial: ~0.5×10⁶ to ~1.0×10⁶

Tempo-iHep3D™ are reprogrammed from human iPSCs, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary serum-free, virus-free, nucleic-acids-free, and integration-free conditions. Tempo-iHep3D™ can be cultured as 2D/monolayer, 3D/spheroids, and multi-cell-type 3D liver organoids. As 3D structures, iHep3D™ can be used for long-term toxicity studies (>25 days in culture).

They express the following biomarkers using RT/q-PCR and Immunocytochemistry methods: AFP, ALB, ASGR1, ASGR2, CYP7A1, CYP2D6, CYP2E1, CYP3A4, CYP2C9, CYP2C19, CYP1A2, BSEP, G6PC, CD36, SLC27A1, HNF4a, Sox17, (and PNPLA3, if co-cultured with Tempo-iHepStellate™).

Intended for scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Long-term storage: liquid nitrogen
Growth Properties: 2D/monolayer cell culture technique; 3D/spheroid/organoid cell culture techniques
QC: Sterility, safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>85%)