Liver sinusoidal endothelial cells (LSECs) are the most abundant non-parenchymal hepatic cell population. They are highly specialized liver endothelial cells that form a barrier to separate hepatocytes and hepatic stellate cells from contacting the blood. In additional to serving as a physical barrier, LSECs have critical physiological and immunological functions. Furthermore, LSECs regulate cell-to-cell crosstalk as chronic liver conditions advance, eventually leading to fibrosis and carcinogenesis.

• Cryovial: ~0.5×10⁶ to ~1.0×10⁶

Tempo-iLSEC™ are reprogrammed from human iPSCs, using integration-free induced pluripotent stem cell (iPSC) lines under a fully defined proprietary serum-free, virus-free, nucleic-acids-free, and integration-free conditions.

They express the following biomarkers using RT/q-PCR and Immunocytochemistry (ICC) methods: LYVE1, MRC1, OIT3, FcGR2B, CLEC4M, KDR, PLPP3, STAB2, CLEC4G, F8, FABP4, FABP5, VWA1, vWF.

Intended for scientific research, drug discovery and therapeutics development use only. It is not a product for human testing or diagnostics.

Long-term storage: liquid nitrogen
Growth Properties: 2D/monolayer cell culture technique; 3D/spheroid/organoid cell culture techniques
QC: Sterility, safety (BioSafety Level 2), HIV/viruses, bacteria, fungi: negative. Cell viability post-thawing (>85%)