CD3e (Clone SK7) is a mouse monoclonal antibody derived from the extracellular domain of the CD3 epsilon chain. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to either a CF® Dye or Astral™ tandem dye. They are supplied in PBS, 0.1% BSA, 0.05% azide.

• Cell/tissue expression: Lymphocytes, T-cells
• Tumor expression: Leukemia/lymphoma
• SwissProt: P07766
• Unigene: 3003
• Positive control: Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60
• Ex/Em: 496, 546, 566/617 nm

CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16 to 28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition.

The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Shelf life: Guaranteed for at least 24 months from date of receipt when stored as recommended.

Type: Primary
Antigen: CD3E
Clonality: Monoclonal
Clone: SK7
Conjugation: RPE-Astral™616
Epitope:
Host: Mouse
Isotype: IgG1 kappa
Reactivity: Human