For quantitative determination of phosphoglucomutase enzyme activity in biological samples.

• Safe and sensitive
• Non-radioactive assay
• Linear detection range in 96-well plate: 0.5 to 20 U/L activity
• Fast and convenient
• The procedure involves addition of a single working reagent and incubation for 20 min
• Room temperature assay
• No 37 °C incubator is needed
• High-throughput
• Homogeneous 'mix-incubate-measure' type assay
• Can be readily automated to assay thousands of samples per day

Phosphoglucomutase (E.C. 5.4.2.2) is an enzyme which catalyzes the interconversion of glucose-1-phosphate (G1P) and glucose-6-phosate (G6P). Phosphoglucomutase (PGM) plays a crucial role in glycogen synthesis and degradation. PGM 1 deficiency can cause a rare congenital disorder of glycosylation. A decrease in PGM activity has also been linked with oxidative stress.

In BioAssay Systems EPGM-100 assay, PGM converts G1P to G6P, which is then oxidized by glucose-6-phosphate dehydrogenase. The formed NADPH is coupled to a formazan chromogen. The increase in absorbance at 460 nm is directly proportional to phosphoglucomutase activity.