Halobacterium salinarum S9 Native Bacteriorhodopsin
Catalog # 77392-866
Supplier: CUBE BIOTECH
CAS Number:
Specifications
- Protein/Peptide Type:Native
- Species:Halobacterium salinarum S9
- Size:500 µg
- Amino Acid Number:262
- Protein Synonyms:BR, HsBR, Bacteriopsin
- Protein/Peptide Name:Bacteriorhodopsin
- Purity:>95%, determined via SDS-PAGE
- Molecular Weight:28256 Da
- Sequence:MLELLPTAVE GVSQAQITGR PEWIWLALGT ALMGLGTLYF LVKGMGVSDP DAKKFYAITT LVPAIAFTMY LSMLLGYGLT MVPFGGEQNP IYWARYADWL FTTPLLLLDL ALLVDADQGT ILALVGADGI MIGTGLVGAL TKVYSYRFVW WAISTAAMLY ILYVLFFGFT SKAESMRPEV ASTFKVLRNV TVVLWSAYPV VWLIGSEGAG IVPLNIETLL FMVLDVSAKV GFGLILLRSR AIFGEAEAPE PSAGDGAAAT SD
- Cat. No.:77392-866
Specifications
About this item
Bacteriorhodopsin (BR) is a 7-transmembrane protein that acts as a light-driven proton pump in archaebacteria.
- Alternative names: Bacteriorhodopsin, BR, HsBR, Bacteriopsin
- UniProt Number: P02945
- Protein class: 7-transmembrane protein
- Organism: Halobacterium salinarum S9
- Sequence, explained: Full-length, wildtype sequence with N-terminal signal sequence which is mostly absent in the preparation
- Affinity tags: None
- Expression host: Halobacterium salinarum S9
- Buffer composition: Purple membrane (PM) patches in H₂O, 0.02% NaN₃
- Purified via: Sequential centrifugation
- Absorbance: Extinction coefficient at 560 nm: 63000 M⁻¹cm⁻¹
- Activity: Binding of ligand all-trans retinal, covalently bound to a lysine residue
- Evaluation of UV-VIS spectrum: absorbance ratio 280/560 nm = 2.3
- Function: Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven proton pump
Due to BR's covalently bound colored ligand, BR is widely used as a control membrane protein, for example for crystallization assays and biochemical/biophysical experiments. We provide BR of high purity, prepared as purple membrane patches from Halobacterium salinarum.