Human Recombinant MSP2N2-His (from E. coli)

Membrane scaffold protein, human origin.

Extinction coefficient (in water) ε280: 44,920 M⁻¹cm⁻¹ (Human origin) 39,880 M⁻¹cm⁻¹ (Mouse origin)
Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTAst
Affinity tag: N-Terminal his-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP2N2 (his-tagged) creates nanodiscs of ~17 nm in diameter.

Note that this products contains a N-terminal his-tag. Keep that in mind when chosing the affinity tag for your membrane protein.

Protein/Peptide Type:
Recombinant
Source:
E. coli
Species:
Human
Storage Conditions:
Storage of lyophilized copolymer: −20 °C for several years; Storage of reconstituted protein: 2...8 °C for several day
Amino Acid Number:
392 (Human origin), 388 (Mouse origin)
Protein Synonyms:
Membrane Scaffold Protein 2N2, His
Protein/Peptide Name:
MSP2N2-His
Purity:
> 90% (Determined by SDS-PAGE)
Molecular Weight:
45670 Da (Human origin), 45526 Da (Mouse origin)
Sequence:
GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ GT PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ
Shipping Temperature:
RT