About this item
PureCube Fe-NTA MagBeads were developed to enrich phosphorylated proteins. They can also be used to purify His-tagged proteins as a secondary function.
- Usage:
- -Enrichment of phosphopeptides
- -Specific binding and purification of 6× His-tagged proteins
- Specificity:
- -Affinity to His-tagged proteins
- -Affinity to phosphorylated biomolecules
- Bead ligand: Fe-NTA
- Bead size: 30 µm
- Filling quantity: Delivered as a 25% suspension
- Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
- pH stability: 2 to 14
- Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Fe-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding a high degree of reproducibility. The phosphopeptide enrichment can be automated.
As an alternative, we also offer PureCube Fe-NTA Agarose resin and said resin pre-packed in cartridges/columns.
To order chemicals, medical devices, or other restricted products please provide ID that includes your business name & shipping address via email [email protected] or fax 484.881.5997 referencing your VWR account number. Acceptable forms of ID are:
- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
Specifications
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Specifications
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