PureCube Fe-NTA MagBeads were developed to enrich phosphorylated proteins. They can also be used to purify His-tagged proteins as a secondary function.

• Usage:
• -Enrichment of phosphopeptides
• -Specific binding and purification of 6× His-tagged proteins
• Specificity:
• -Affinity to His-tagged proteins
• -Affinity to phosphorylated biomolecules
• Bead ligand: Fe-NTA
• Bead size: 30 µm
• Filling quantity: Delivered as a 25% suspension
• Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
• pH stability: 2 to 14
• Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Fe-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding a high degree of reproducibility. The phosphopeptide enrichment can be automated.

As an alternative, we also offer PureCube Fe-NTA Agarose resin and said resin pre-packed in cartridges/columns.