PureCube 100 Ni-INDIGO agarose resin is a unique development by Cube Biotech for His-tag protein purifications of the highest quality.

• Usage: Specific binding and purification of 6× His tagged proteins
• Specificity: Affinity to His tagged proteins
• Binding capacity: >100 mg/ml
• Bead ligand: Ni-INDIGO
• Bead size:100 μm
• Chelator stability: Stable in buffer containing 20 mM DTT and 20 mM EDTA
• Filling quantity: Delivered as a 50% suspension

Developed with the aim in mind of creating an agarose resin that can withstand the highest concentrations of chelators like EDTA and DTT, which are common in mammalian cell culture buffers. The resulting INDIGO ligand has both DTT and EDTA tolerances of 20 mM each. That is highly superior to other competing resins.

In biotechnological and pharmaceutical processes in general, the INDIGO-Ni ligand has the advantage over the NTA ligand that no nickel leakage occurs due to its strong binding.

Ni-INDIGO is also available as magnetic beads or as pre-packed in cartridges/columns.

Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents and equipment optional: Western Blot reagents and equipment