PureCube NTA XL Agarose

PureCube NTA XL (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods.

Usage: For coupling of metal ions for corresponding purification and/or enrichment purposes
Specificity: Varies
Binding capacity: Depends on the loaded ion
Purity: Depends on the loaded ion
Bead ligand: NTA
Bead size: 400 μm
Matrix: 7.5% cross-linked agarose
Filling quantity: Delivered as a 50% suspension
Recommended FPLC flow rates: 0.5 to 2 ml/min
Maximal FPLC flow rate: 6 ml/min
pH stability: 2 to 14
Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

The beads in this suspension have an average diameter of 400 µm. This size is relatively large, which is helpful for very high flow rates in, e.g., FPLC assays or very viscous cell media. Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can, in turn, attach your desired metal ions to the agarose beads.

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