PureCube Ni-NTA agarose resins are your best option to purify His-tagged protein using FPLC, Batch spin, or column chromatography.

• Usage: Specific binding and purification of 6× His tagged proteins
• Specificity: Affinity to His tagged proteins
• Binding capacity: 20 to 80 mg/ml, depending on bead size
• Bead ligand: Ni-NTA
• Bead size: 40 μm, 100 µm, or 400 µm
• Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
• Filling quantity: Delivered as a 50% suspension

PureCube Ni-NTA agarose size ranges from market standard 40 µm and 100 µm diameters to 400 µm (XL). These three options provide plenty of possibilities to choose the correct agarose resin for your purpose. Choose smaller beads for higher mechanical stability and higher protein yield. Meanwhile, bigger beads provide faster flow rates and the possibility of working with very viscous cell media.

Required equipment:
-Lysis buffer
-Wash buffer
-Elution buffer
-Ice bath
-Refrigerated centrifuge for 50 ml tube (min 10000×g)
-50 ml centrifuge tube
-Micropipettor and Micropipetting tips
-Disposable gravity flow columns with capped bottom outlet, 2 ml
-pH meter
-End-over-end shaker
-SDS-PAGE buffers, reagents, and equipment; Optional: Western Blot reagents and equipment