Nanodisc MSP2N2-His DMPC

This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.

• Origin of the MSP protein: Human
• Purity:>90% (Determined by SDS-PAGE)
• Number of amino acids: 392
• Molecular mass: 45670 Da
• Extinction coefficient (in water) ε280: 44920 M⁻¹cm⁻¹
• Buffer: 20 mM TRIS pH 7.4, 100 mM NaCl, 0.5 mM EDTA
• Sequence (1-Letter code): GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ GT PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ
• Affinity tag: N-Terminal His-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP2N2 (His-tagged) creates nanodiscs of ∼17 nm in diameter.

This product contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.

Delivery information: Shipping temperature: Dry-ice; Storage of reconstituted protein: –80 °C, avoid freeze-thaw cycle.