Nanodisc Assembly Kit MSP1D1 POPC

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.

• Purity: >90% (determined by SDS-PAGE)
• Number of amino acids: 277
• Molecular mass: 32600 Da
• Extinction coefficient (in water) ε280: 29400 M⁻¹ cm⁻¹
• Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
• Sequence (Human origin): GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ
• Affinity tag: N-Terminal His-tag

Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences to stabilize membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 creates nanodiscs of ∼9 to 10 nm in diameter.

With this assembly kit both cellular as well as cell-free expressed proteins can be stabilized in MSP nanodiscs.

Delivery information: Shipping temperature: Room temperature; storage of lyophilized copolymer: −20 °C for several years; storage of reconstituted protein: 2 - 8 °C for several day.