PureCube Ti-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.
- Usage: Specific binding and purification of 6× His-tagged proteins, binding of copper-binding proteins
- Specificity: Affinity to His-tagged proteins, affinity to copper-binding
- Binding capacity: >80 mg/ml
- Bead Ligand: Cu-NTA
- Bead size: 40 μm
- Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
- Filling quantity: Delivered as a 50% suspension
Required equipment and materials:
- FPLC hardware like e.g. AKT
- Binding buffer
- Elution buffer
The affinity matrix is based on 6% cross-linked agarose that was magnetized. The bead ligand is Ti-NTA and the particle diameter is 30 µm. They are very homogeneous in size, yielding in a high degree of reproducibility. The phosphopeptide enrichment with Cube Biotech MagBeads can be automated.
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- • State issued document with your organization's Federal Tax ID Number
- • State issued document with your organization's Resale Tax ID Number
- • City or County issued Business License
- • State Department of Health Services License
- • Any other ID issued by the State that includes the business name & address
* ATTN: California Customers may require additional documentation as part of the CA Health & Safety Code. Products that fall under this regulation will be placed on a mandatory 21-day hold after documentation is received. Avantor will not lift restrictions for residential shipping addresses.
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