PureCube NHS (N-hydroxy succinimide) agarose XL resin is your best option to couple biomolecules carrying free-standing amine groups.

• Usage: Coupling proteins to active maleimide groups
• Specificity: Exposed thiol (SH) groups on biomolecule
• Bead ligand: Chemically active maleimide groups; resulting bond; irreversible Thioether
• Protein binding capacity: ≥15 mg/ml
• Bead size: 400 μm
• Filling quantity: Delivered as a 50% suspension
• Matrix: 7.5% cross-linked agarose
• Short term storage: In neutral buffer at 4 °C
• Long term storage: 100 mM sodium hydrogen carbonate, 0.02% sodium azide, pH 7.5 at 4 °C or 20 mM sodium acetate, 20% ethanol, pH 6.5 at 4 °C
• Shelf life: If properly stored - three months upon delivery, unopened
• Chemical structure: Maleimide structure

The resulting amide bond between the NHS Agarose beads and your biomolecule of interest is stable so that the bead can be used for other applications afterward. Our PureCube NHS activated agarose is also available as magnetic beads if you prefer this purification method or as agarose resin with smaller beads if you need higher mechanical stability of the beads.