Our PureCube octyl agarose resin is a suspension of purification beads for Hydrophobic Interaction Chromatography (HIC).

• Antibody name: PentaHis antibody, BSA-free
• Cell Line: Mouse, hybridoma
• Suitable for: Western blot, ELISA
• Detection limit Western blot, chromogenic detection: 2 ng
• Detection limit Western blot, chemiluminescent detection: 50 pg
• Quantity: 100 µg
• Physical state: Dried lyophilized powder
• Reconstitution of lyophilized antibody: Add 500 µl ddH₂O to obtain a solution of 0.2 mg/ml concentration
• Recommended working dilution: 1:1000
• Shipment temperature: Shipped at ambient temperature
• Short-term storage: After resuspension, store vial at 2 to 8 °C for up to 3 months. Centrifuge product if not clear after standing at room temperature and/or heating to 37 °C
• Long-term storage: Store as lyophilized powder for up to 12 months at 2 to 8 °C. Alternatively, aliquot contents and freeze at –20 °C for up to six months. Avoid repeated freeze-thaws

Hydrophobic Interaction Chromatography (HIC) is a reliable method to separate proteins and other biomolecules based on their hydrophobicity. If you use HIC with your sample for the first time we recommend testing all of our HIC resins before.